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Genome_build: Raw sequence reads were mapped to the A. Learn more about QIAGEN CLC Genomics Workbench. Additionally, it includes all the sequence analysis tools of CLC Main Workbench. 1997) was used in modelling, and CLC sequence viewer (CLC bio, a QIAGEN. Supports all major next-generation sequencing platforms Product Details CLC Genomics Workbench is a comprehensive analysis package for the analysis and visualization of data and supports all typical NGS workflows. Pairwise comparison of the expression results was performed using the total mapping results for deletion mutants vs wild type.ĭifferential gene lists were created with 2-fold expression cutoff and significant p-values of <0.05 to identify genes that were up- or down-regulated under each condition. They encoded the generation sequencing data supporting the results of this.
#Qiagen clc sequence viewer software#
Raw data for each sample was analyzed using CLC Genomics Workbench version 12.0.1 software (QIAGEN Aarhus, Denmark), with default settings, based on their proprietary algorithms. CLC Main Workbench is a professional software application specialized in advanced protein sequence analyses, data management, as well visual. RNA libraries were prepared for sequencing using Illumina protocols Review by Ana Marculescu on September 11, 2014. Libraries of depleted RNA were prepared using the Illumina TruSeq RNA LT v2 kit according to the manufacturer's instructions. Samples with the amount at least 0.5-1 µg were used for library construction and RNA sequencing. Final concentrations and purity grades of the samples were determined using Bioanalyzer 2100 (Agilent, USA).
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High quality total RNA was further processed by removing 23S and 16S rRNAs using the MicrobExpress bacterial mRNA enrichment kit (Life Technologies, USA). Total RNA was isolated from cells using hot phenol method. GEO help: Mouse over screen elements for information.Ĭells were grown in LB broth until mid-exponential phase (OD600 = 0.5-0.6)